Crystalline beta-Glucuronidase.

Application of an improved glucuronidase assay method to the study of human blood beta-glucuronidase.

Previous conditions in which phenolphthalein glucuronide was employed as substrate (1) have proved unsatisfactory for the determination of glucuronidase in plasma, serum, and laked blood cells. Thus, low glucuronidase activities such as frequently occur in plasma could not be accurately measured or even approximated, owing to a combination of circumstances; i.e., too short an incubation period ...

Lumenal location of the microsomal beta-glucuronidase-egasyn complex

Mouse liver beta-glucuronidase is stabilized within microsomal vesicles by complexation with the accessory protein egasyn. The location of the beta-glucuronidase-egasyn complex and free egasyn within microsomal vesicles was investigated. Surprisingly, it was found that neither the complex nor free egasyn are intrinsic membrane components. Rather, both are either free within the vesicle lumen or...

Beta-glucuronidase activity in human female genital cancer.

In 1937 Marrian (1) described a reaction where by estriol glucuronide was hydrolyzed during its passage through mouse intestine. This action was ascribed to a $-glucuronidase.' In a series of ex periments, Fishman (2) and others (3) associated the activity of this enzyme more closely with the metabolism of glucuronic acid. The glucuronidase activity of mouse liver was increased following the ad...

The lymphocyte beta-glucuronidase activity in lymphoproliferative disorders.

Colorimetric enumeration of Escherichia coli based on beta-glucuronidase activity.

A medium containing a chromogenic substrate was developed for the enumeration of Escherichia coli on the basis of beta-glucuronidase activity. In this medium there was an inverse linear relationship between the log initial E. coli concentration and the time taken for the color to reach a threshold optical density of 0.05. This relationship applied even when the E. coli population contained 5% b...