Crystal Structures and Inhibition Kinetics Reveal a Two-Stage Catalytic Mechanism with Drug Design Implications for Rhomboid Proteolysis

Mechanism of intramembrane proteolysis investigated with purified rhomboid proteases.

Intramembrane proteases have the unusual property of cleaving peptide bonds within the lipid bilayer, an environment not obviously suited to a water-requiring hydrolysis reaction. These enzymes include site-2 protease, gamma-secretase/presenilin, signal peptide peptidase and the rhomboids, and they have a wide range of cellular functions. All have multiple transmembrane domains and, because of ...

investigating the feasibility of a proposed model for geometric design of deployable arch structures

deployable scissor type structures are composed of the so-called scissor-like elements (sles), which are connected to each other at an intermediate point through a pivotal connection and allow them to be folded into a compact bundle for storage or transport. several sles are connected to each other in order to form units with regular polygonal plan views. the sides and radii of the polygons are...

Crystal structure of biotin carboxylase in complex with substrates and implications for its catalytic mechanism.

Biotin-dependent carboxylases are widely distributed in nature and have important functions in many cellular processes. These enzymes share a conserved biotin carboxylase (BC) component, which catalyzes the ATP-dependent carboxylation of biotin using bicarbonate as the donor. Despite the availability of a large amount of biochemical and structural information on BC, the molecular basis for its ...

Two high-resolution crystal structures of potato 1,3-β-glucanase reveal subdomain flexibility with implications for substrate binding

Multi-Objective Learning Automata for Design and Optimization a Two-Stage CMOS Operational Amplifier

In this paper, we propose an efficient approach to design optimization of analog circuits that is based on the reinforcement learning method. In this work, Multi-Objective Learning Automata (MOLA) is used to design a two-stage CMOS operational amplifier (op-amp) in 0.25μm technology. The aim is optimizing power consumption and area so as to achieve minimum Total Optimality Index (TOI), as a new...