Cryptosporidiosis Modulates the Gut Microbiome and Metabolism in a Murine Infection Model

Cryptosporidiosis is a major human health concern globally. Despite well-established methods, misdiagnosis remains common. Our understanding of the cryptosporidiosis biochemical mechanism limited, compounding difficulty clinical diagnosis. Here, we used systems biology approach to investigate underlying interactions in C57BL/6J mice infected with Cryptosporidium parvum. Faecal samples were collected daily following infection. Blood, liver tissues and luminal contents 10 days post High-resolution liquid chromatography low-resolution gas coupled mass spectrometry analyse proteomes metabolomes these samples. Faeces additionally subjected 16S rRNA gene sequencing. Univariate multivariate statistical analysis acquired data illustrated altered host microbial energy pathways during Glycolysis/citrate cycle metabolites depleted, while short-chain fatty acids D-amino accumulated. An increased abundance bacteria associated stressed gut environment was seen. Host proteins involved Lactobacillus glyceraldehyde-3-phosphate dehydrogenase upregulated cryptosporidiosis. Liver oxalate also Microbiome–parasite relationships observed be more influential than host–parasite association mediating changes mouse Defining this parasite–microbiome interaction first step towards building comprehensive model biomarker discovery, rapid accurate diagnostics.