Cryopreservation of Winter-dormant Apple Buds Using Two-step Freezing
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Vegetative Dormant Winter Buds
Cryopreservation of vegetative winter buds is an alternative method to preserving plant species in the field. In this method, nodal sections of scionwood are stored in liquid nitrogen vapor. Cryopreservation of the vegetative dormant winter buds involves low maintenance costs and virtually eliminates the risk of germplasm loss. At the NCGRP, this method is routinely used to preserve Malus sp. (...
متن کاملFreezing of water in dormant vegetative apple buds in relation to cryopreservation.
Various empirical prefreezing protocols have been used to facilitate cryopreservation of dormant buds from woody plants. The objective of this research was to determine the quantity of water remaining in liquid phase, under different prefreezing conditions using pulsed nuclear magnetic resonance spectroscopy of dormant apple (Malus domestica Mill.) buds from three cultivars. During prefreezing,...
متن کاملCryopreservation of winter-dormant apple buds: II - tissue water status after desiccation at -4C and before further cooling.
The established protocol for the cryopreservation of winter-dormant Malus buds requires that stem explants, containing a single, dormant bud are desiccated at -4 degree C, for up to 14 days, to reduce their water content to 25-30 percent of fresh weight. Using three apple cultivars, with known differences in response to cryopreservation, the pattern of evaporative water loss has been characteri...
متن کاملCryopreservation of dormant buds from diverse Fraxinus species.
Ash (Fraxinus) is an economically important tree genus in the landscape industry, as well as a key component of North American forests, especially in the North Central United States and adjacent regions in Canada. In recent years, the Emerald Ash Borer beetle (Agrilus planipennis) has significantly threatened the survival of native North American Fraxinus species. A dormant-bud cryopreservation...
متن کاملGenetic stability of mulberry germplasm after cryopreservation by two-step freezing technique
Genetic stability of Morus alba, Morus indica, Morus laevigata (indigenous collection) and Morus species (exotic collection) have been studied in in vitro regenerated plants of mulberry (fresh, before and after cryopreservation) using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. This study examined the genetic stability of cryopreserved dormant buds o...
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ژورنال
عنوان ژورنال: Plant Breeding and Biotechnology
سال: 2013
ISSN: 2287-9366
DOI: 10.9787/pbb.2013.1.3.283