CRYOPRESERVATION OF DORMANT APPLE (Malus sp.) BUDS.
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Vegetative Dormant Winter Buds
Cryopreservation of vegetative winter buds is an alternative method to preserving plant species in the field. In this method, nodal sections of scionwood are stored in liquid nitrogen vapor. Cryopreservation of the vegetative dormant winter buds involves low maintenance costs and virtually eliminates the risk of germplasm loss. At the NCGRP, this method is routinely used to preserve Malus sp. (...
متن کاملFreezing of water in dormant vegetative apple buds in relation to cryopreservation.
Various empirical prefreezing protocols have been used to facilitate cryopreservation of dormant buds from woody plants. The objective of this research was to determine the quantity of water remaining in liquid phase, under different prefreezing conditions using pulsed nuclear magnetic resonance spectroscopy of dormant apple (Malus domestica Mill.) buds from three cultivars. During prefreezing,...
متن کاملCryopreservation of dormant buds from diverse Fraxinus species.
Ash (Fraxinus) is an economically important tree genus in the landscape industry, as well as a key component of North American forests, especially in the North Central United States and adjacent regions in Canada. In recent years, the Emerald Ash Borer beetle (Agrilus planipennis) has significantly threatened the survival of native North American Fraxinus species. A dormant-bud cryopreservation...
متن کاملCryopreservation of winter-dormant apple buds: II - tissue water status after desiccation at -4C and before further cooling.
The established protocol for the cryopreservation of winter-dormant Malus buds requires that stem explants, containing a single, dormant bud are desiccated at -4 degree C, for up to 14 days, to reduce their water content to 25-30 percent of fresh weight. Using three apple cultivars, with known differences in response to cryopreservation, the pattern of evaporative water loss has been characteri...
متن کاملCryopreservation of Populus trichocarpa and Salix dormant buds with recovery by grafting or direct rooting.
BACKGROUND Methods are needed for the conservation of clonally maintained trees of Populus and Salix. In this work, Populus trichocarpa and Salix genetic resources were cryopreserved using dormant scions as the source explant. OBJECTIVE We quantified the recovery of cryopreserved materials that originated from diverse field environments by using either direct sprouting or grafting. MATERIAL...
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ژورنال
عنوان ژورنال: HortScience
سال: 1990
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.25.9.1073f