Cryopreservation of common carp sperm

Cryopreservation of common carp (Cyprinus carpio) embryos using different cryoprotectant

This study reports that common carp (Cyprinus carpio) embryos were successfully frozen and preserved by vitrification. The modified Haga solution was tested by using two different cryoprotectants and three different concentrations at 4°C to obtain appropriate vitrified solutions ((dimethylsulfoxide 5, 10, 15% (DMSO) and glycerol 5, 10, 15% (gly)) in the study.  The hatching rates of embryos exp...

Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C

In the present study, cryopreservation of common carp (Cyprinus carpio) embryos was studied using a stepwise cooling protocol. Embryos at heartbeat stage were selected, placed in 15- ml plastic tubes and stored at -2 °C for 1, 7, 14, and 21 days in 2 different cryopreservation solutions: S1 (methanol + dimethyl sulfoxide + sucrose) and S2 (methanol + propylene glycol + sucrose). Embryo viabilit...

Cryopreservation of Rat Sperm

The laboratory rat, Rattus norvegicus, was the first mammalian species domesticated for scientific research, which work dating back to before 1850. From this auspicious beginning, the rat has become the most widely studied experimental animal model for biomedical research (Jacob, 1999). Since the development of the first inbred rat strain by King 1909, over 500 inbred rat strains have been deve...

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Sperm Production and Cryopreservation in Muskellunge after Carp Pituitary Extract and Human Chorionic Gonadotropin Injection

— We investigated the effects of carp pituitary extract (CPE) and human chorionic gonadotropin (hCG) on the sperm production in muskellunge Esox masquinongy. Total volumes of milt collected from fish (mean weight, 4.8 ± 1.5 kg) injected with CPE, hCG, or the saline control were 5.36 ± 3.75 mL, 3.1 ± 1.52 mL, and 3.89 ±2.16 mL, respectively. Sperm concentration, protein and mineral concentration...