Cryopreservation of cells using defined serum-free cryoprotective agents
نویسندگان
چکیده
منابع مشابه
Serum-free culturing of mammalian cells--adaptation to and cryopreservation in fully defined media.
Long term storage of living cells is a central issue in cell biology and medicine. In addition to the cryoprotectant dimethyl sulphoxide (DMSO), foetal bovine serum (FBS) is often added to the freezing medium for the cryoconservation of serum dependent cell lines. FBS, with its high protein content, protects cells against shear forces and gives the medium a desirable osmotic environment with a ...
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ژورنال
عنوان ژورنال: Current Directions in Biomedical Engineering
سال: 2016
ISSN: 2364-5504
DOI: 10.1515/cdbme-2016-0070