Crossed Immunoelectrophoresis of Platelets

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Crossed immunoelectrophoresis of human platelet membranes.

Human platelet membranes were prepared by the glycerol-lysis technique of Barber and Jamieson (Barber, A. J., and Jamieson, G. A. (1970) J. Biol. Chem 245, 6357-6365), solubilized in 1% Triton X-100 and subjected to crossed immunoelectrophoresis, in 1% agarose, employing a rabbit anti-human platelet membrane antibody in the second dimension. Approximately 20 immunoprecipitates (numbered 1 to ...

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ژورنال

عنوان ژورنال: Japanese Journal of Thrombosis and Hemostasis

سال: 1990

ISSN: 1880-8808,0915-7441

DOI: 10.2491/jjsth.1.269