Cross Species use of Human Microarray Genotyping Technology for Bornean Orangutan (Pongo pygmaeus) SNP Discovery

Previous genetic studies of orangutans (Pongo spp.) have relied mainly upon mitochondrial DNA or microsatellite short tandem repeats (STR) for genomic genotyping analysis. Scientists yet to take advantage the closeness great apes humans analysis by using advanced techniques available human genotyping. To genotype at Tanjung Puting National Park, we developed a novel combination methyl-based magnetic enrichment capture fecal with on targeted single nucleotide polymorphism (SNP) microarray, and compared this additional micro-capillary We successfully isolated 125 known SNP loci (0.08% those targeted) which hybridized orangutan Illumina Infinium QC array. estimated diversity relatedness (r) three estimators total 32 (21 female 9 male) wild Camp Leakey study site. Average TrioML within sample, from our combo SNP/STR dataset, was range consistent half first cousins (r = .082). All sampled males females had relatives site indicating verified local, closely related community Leakey.