Cross-Reactivity Pattern of Asian and American Human Gnathostomiasis in Western Blot Assays Using Crude Antigens Prepared from Gnathostoma spinigerum and Gnathostoma binucleatum Third-Stage Larvae
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چکیده
منابع مشابه
Ultrastructural effects of albendazole on the body wall of Gnathostoma spinigerum third stage larvae.
This study investigated the effects of albendazole (ABZ) on the ultrastructure of Gnathostoma spinigerum advanced third-stage larvae. Two groups of experimentally infected mice received 60 or 90 mg/kg ABZ orally once a day for 21 consecutive days. Both groups had damage to the body walls of the worms, especially to the non-contractile part of the muscular layer. The severity of the damage was d...
متن کاملFeasibility Of Using Orange Juice For Digestion Of Swamp Eel Viscera For Harvesting Infective Stage Larva Of Gnathostoma Spp
Gnathostoma spinigerum third stage larvae antigen is necessary for Western blot analysis in the diagnosis of Gnathostomiasis. Acid pepsin solution is required for digestion of eel's liver( Fluta alba ) to yield the larvae for antigen preparation. Here, he reported the feasibility of using orange juice as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third stage larvae. Fr...
متن کاملPrevalence and intensity of third stage Gnathostoma spinigerum larvae in swamp eels sold in three large markets in Bangkok, Thailand.
Gnathostoma spinigerum is a common human tissue parasite in Thailand. The swamp eel is the major intermediate or paratenic host for this parasite. The high prevalence of the infective third stage larvae (L3) of this parasite in the rainy season has been noted in previous studies. During June 1999 (rainy season), we performed a cross-sectional survey of the prevalence and the intensity of G. spi...
متن کاملSpecific IgE antibody responses to somatic and excretory-secretory antigens of third stage G. spinigerum larvae in human gnathostomiasis.
Specific IgE antibody levels in the serum of patients with proven gnathostomiasis and in those with intermittent cutaneous migratory swelling (CMS) were determined by the enzyme-linked immunosorbent assay (ELISA) using somatic extract and excretory-secretory (ES) products of Gnathostoma spinigerum infective larvae as antigens. The third stage larval used were obtained from naturally infected ee...
متن کاملIntraocular Gnathostoma spinigerum: a case report
Live intraocular nematode is a rare occurrence that is mostly reported in South East Asian countries. Herewith we report such a case from Nayagarh district of Odisha. A 28 year old female presented with swelling, redness, lacrimation, pain & diminished vision of left eye since 2 1/2 years. Slit lamp examination revealed a worm piercing iris muscle. The worm was removed by paracentesis of anteri...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2016
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.16-0176