Cross-genera amplification and identification of Colpodella sp. with Cryptosporidium primers in fecal samples of zoo felids from northeast China

Abstract The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification protozoan considered a more reliable and rapid method for than microscopy. Among protozoans, Cryptosporidium being one important water-borne zoonotic major cause diarrheal disease named cryptosporidiosis in humans, domestic animals, wild animals. This study was aimed identify zoo felids (N= 56) belonging different China, but accidentlly Colpodella encountered sample phylogenetic data confirmed this unexpected amplification from fecal samples using two-step nested-PCR. Phylogenetic analysis revealed fact about specific primers used previously by researchers cross-genera amplification. We came know that genetically sequenced amplicon gives accurate species. suggests investigation on which has been neglected gains attention after identified humans animals known correlate with neurological symptoms patients.