CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Gemmae from the Liverwort Marchantia polymorpha L.
The liverwort Marchantia polymorpha L. is one of the key model plants in evo-devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of M. polymorpha plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, t...
متن کاملHomologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.
The liverwort Marchantia polymorpha is an emerging model organism on account of its ideal characteristics for molecular genetics in addition to occupying a crucial position in the evolution of land plants. Here we describe a method for gene targeting by applying a positive/negative selection system for reduction of non-homologous random integration to an efficient Agrobacterium-mediated transfo...
متن کاملAgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia polymorpha L.
The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). ...
متن کاملAgrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology.
Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sp...
متن کاملThe Elegant Simplicity of the Liverwort Marchantia polymorpha.
Plants have evolved several ways to remain dormant until the time is right for growth. For example, seeds maintain dormancy through the antagonistic effects of the phytohormones abscisic acid (ABA) and gibberellic acid. Auxin (indole-3-acetic acid [IAA]), in turn, regulates ABA-induced seed dormancy through its interaction with the ABA signaling pathway (Liu et al., 2013). Dormancy is also cruc...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2014
ISSN: 1471-9053,0032-0781
DOI: 10.1093/pcp/pcu014