CRISPR Spacers Indicate Preferential Matching of Specific Virioplankton Genes
نویسندگان
چکیده
منابع مشابه
Optimal number of spacers in CRISPR arrays
Prokaryotic organisms survive under constant pressure of viruses. CRISPR-Cas system provides its prokaryotic host with an adaptive immune defense against viruses that have been previously encountered. It consists of two components: Cas-proteins that cleave the foreign DNA and CRISPR array that suits as a virus recognition key. CRISPR array consists of a series of spacers, short pieces of DNA th...
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Clustered regularly interspaced short palindromic repeats (CRISPR) in bacterial and archaeal DNA have recently been shown to be a new type of anti-viral immune system in these organisms. We here study the diversity of spacers in CRISPR under selective pressure. We propose a population dynamics model that explains the biological observation that the leader-proximal end of CRISPR is more diversif...
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Clustered regularly interspaced short palindromic repeat (CRISPR) systems are the adaptive immune systems of bacteria and archaea against viral infection. While CRISPRs have been exploited as a tool for genetic engineering, their spacer sequences can also provide valuable insights into microbial ecology by linking environmental viruses to their microbial hosts. Despite this importance, metageno...
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Two algorithmic results are presented that are pertinent to the matching of patterns typically used by biologists to describe regions of macromolecular sequences that encode a given function. The first result is a threshold-sensitive algorithm for approximately matching both network and regular expressions. Network expressions are regular expressions that can be composed only from union and con...
متن کاملCreation and Analysis of a Virome: Using CRISPR Spacers.
Advances in sequencing technology have allowed for the study of complex and previously unexplored microbial and viral populations; however, linking host-phage partners using in silico techniques has been challenging. Here, we describe the flow-through for creation of a virome, and its subsequent analysis with the viral assembly and analysis module "Viritas," which we have recently developed. Th...
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ژورنال
عنوان ژورنال: mBio
سال: 2019
ISSN: 2161-2129,2150-7511
DOI: 10.1128/mbio.02651-18