Creation of recombinant Chlamydomonas reinhardtii strains expressing codon optimized vp28 gene from white spot symdrome virus
نویسندگان
چکیده
منابع مشابه
Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate
White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein...
متن کاملMolecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate
White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein...
متن کاملmolecular cloning and recombinant expression of the vp28 (wsv421 gene) from iranian white spot syndrome virus isolate
white spot syndrome virus (wssv) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including iran. in the present study, a pair of primers was designed according to the sequence of vp 28 gene of wssv in the genbank. vp28 gene from an iranian wssv isolate (irvp28) was cloned, sequenced and expressed in escherichia coli bl21(de3) strain in order to produce vp28 protein...
متن کاملInvestigation of an Optimized Context for the Expression of GFP as a Reporter Gene in Chlamydomonas Reinhardtii
Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. The Green Fluorescent Protein (G...
متن کاملThe utilization of VP28 gene to protect penaeid shrimps from white spot syndrome virus disease: a review
White spot syndrome (WSS) is a viral disease which affects most of the commercially cultivated marine shrimp species all over the world causing significant losses. Deaths occur within days of symptoms and affect a diverse range of commercial crustacean species. A heavy mortality is usually observed in the entire infected population. Survival rates can be anywhere from 30% to zero. Consequently,...
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ژورنال
عنوان ژورنال: TAP CHI SINH HOC
سال: 2018
ISSN: 0866-7160,0866-7160
DOI: 10.15625/0866-7160/v40n1.10988