Covalent Small-Molecule-RNA Complex Formation Enables Cellular Profiling of Small-Molecule-RNA Interactions

Defining RNA–Small Molecule Affinity Landscapes Enables Design of a Small Molecule Inhibitor of an Oncogenic Noncoding RNA

RNA drug targets are pervasive in cells, but methods to design small molecules that target them are sparse. Herein, we report a general approach to score the affinity and selectivity of RNA motif-small molecule interactions identified via selection. Named High Throughput Structure-Activity Relationships Through Sequencing (HiT-StARTS), HiT-StARTS is statistical in nature and compares input nucl...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Small hairpin RNA as a small molecule sensor

Life has perfected the art of connecting the myriad molecular components in a live cell through regulatory links, transforming a cell from a mere protein shake into the most complex physical system known to man. Research in synthetic biology has strived, among other things, to recreate this complexity to a modest degree. This requires building new regulatory links and networks according to pre-...

Small Molecule Therapeutics Small Molecule BMH-Compounds That Inhibit RNA Polymerase I and Cause Nucleolar Stress

Activation of the p53 pathway has been considered a therapeutic strategy to target cancers. We have previously identified several p53-activating small molecules in a cell-based screen. Two of the compounds activated p53 by causing DNA damage, but this modality was absent in the other four. We recently showed that one of these, BMH-21, inhibits RNA polymerase I (Pol I) transcription, causes the ...

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...