Counting the Proteins in Plasma

Counting the proteins in plasma.

Featured Article: Anderson NL, Polanski M, Pieper R, Gatlin T, Tirumalai RS, Conrads TP, et al. The human plasma proteome: a non-redundant list developed by combination of four separate sources. Mol Cell Proteomics 2004;3:311–26. The problem of enumerating protein components in plasma has challenged the best analytical technologies for more than 80 years. The first generation of proteomics meth...

The plasma Proteins

Automated Subunit Counting of Membrane Proteins 1 AUTOMATING SINGLE SUBUNIT COUNTING OF MEMBRANE PROTEINS IN MAMMALIAN CELLS*

Background: Although powerful, single subunit counting is time consuming, prone to user-bias and largely restricted to Xenopus expression. Results: PIF is an automated analysis program that identifies subunit stoichiometry of any fluorescently-tagged membrane protein from TIRF-recordings. Conclusion: PIF is accurate to more than 90% even in noisy data typical for mammalian expression system. Si...

The Iranian Blood Transfusion, Donor Safety Program: Effect of Long-term Plasmapheresis on Plasma Proteins

Background: Plasmapheresis is a well-recognised method for harvestingplasma.  It was introduced in the 1950s and is currently employed worldwide.  This method has been in use in Iran since 1979. Objective: To evaluate the efficacy of donor safety program used at the Iranian Blood Transfusion Organization. Methods: A comparison is made of the protein concentrations between the first-time and lon...

the u.s. policy in central asia and its impact on the colored revolutions in the region (the case study of tulip revolution in kyrgyzstan)

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