Correlative Tomography

Correlative Tomography

Increasingly researchers are looking to bring together perspectives across multiple scales, or to combine insights from different techniques, for the same region of interest. To this end, correlative microscopy has already yielded substantial new insights in two dimensions (2D). Here we develop correlative tomography where the correlative task is somewhat more challenging because the volume of ...

Correlative Array Tomography – from 2D towards 3D

Correlative microscopy combines information from light and electron microscopy (LM and EM) into one comprehensive dataset. The “Shuttle & Find” interface from Zeiss was the first easy to use solution for imaging one and the same sample region in different microscopes, i.e. from widefield or confocal LMs to environmental or field emission SEMs (FE-SEM). However, Shuttle & Find is limited to 2D a...

Projection Neuron Circuits Resolved Using Correlative Array Tomography

Assessment of three-dimensional morphological structure and synaptic connectivity is essential for a comprehensive understanding of neural processes controlling behavior. Different microscopy approaches have been proposed based on light microcopy (LM), electron microscopy (EM), or a combination of both. Correlative array tomography (CAT) is a technique in which arrays of ultrathin serial sectio...

FIB Preparation of Bone–Implant Interfaces for Correlative On-Axis Rotation Electron Tomography and Atom Probe Tomography

Osseointegration refers to the direct bonding between titanium implants and bone, making it one of the key factors for the success of reconstructive and regenerative orthopaedic and dental surgeries [1]. Resolving the implantbone interface with high chemical or spatial resolution enables a clearer determination of bone growth mechanisms at the implant interface and optimal modification of impl...

Correlative Neuroanatomy