Correlative Fluorescence and Electron Microscopy in 3D
نویسندگان
چکیده
منابع مشابه
Simultaneous Correlative Scanning Electron and High-NA Fluorescence Microscopy
Correlative light and electron microscopy (CLEM) is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with electron microscopy. Widespread application of correlative microscopy is hampered by elaborate experimental procedures related foremost to retrieving ...
متن کاملCorrelative Fluorescence- and Scanning, Transmission Electron Microscopy for Biomolecular Investigation
These days, cellular and molecular biology are closely integrated due to the development of novel correlative biomolecular microscopy platforms, even though this avant-garde vision of combining different imaging tools has been around since the late 1960s. The current boom and successful application of correlative imaging methods to dissect the cell’s internal structure is progressing faster tha...
متن کاملCorrelative 3D superresolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes.
Microscopic images of specific proteins in their cellular context yield important insights into biological processes and cellular architecture. The advent of superresolution optical microscopy techniques provides the possibility to augment EM with nanometer-resolution fluorescence microscopy to access the precise location of proteins in the context of cellular ultrastructure. Unfortunately, eff...
متن کاملStochastic Micro-Pattern for Automated Correlative Fluorescence - Scanning Electron Microscopy
Studies of cellular surface features gain from correlative approaches, where live cell information acquired by fluorescence light microscopy is complemented by ultrastructural information from scanning electron micrographs. Current approaches to spatially align fluorescence images with scanning electron micrographs are technically challenging and often cost or time-intensive. Relying exclusivel...
متن کامل3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy
The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastru...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2017
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927617007073