Correction: Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo

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Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo

Clostridium difficile is the principal cause of antibiotic-associated diarrhea. Major metabolic requirements for colonization and expansion of C. difficile after microbiota disturbance have not been fully determined. In this study, we show that glutamate utilization is important for C. difficile to establish itself in the animal gut. When the gluD gene, which codes for glutamate dehydrogenase (...

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Evaluation of an Enzyme Immunoassay for Detection of the Clostridium difficile Common Antigen, Glutamate Dehydrogenase (GDH), in Fecal Specimens

C. difficile is reported to cause up to 25% of cases of antibiotic-associated diarrhea (AAD) and most cases of pseudomembranous colitis (PMC). Laboratory diagnosis typically consists of toxin detection in fecal specimens by enzyme immunoassay (EIA) or tissue culture cytotoxin assay (CTA). Culture on C. difficile selective media offers the highest sensitivity, but has low specificity due to dete...

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Glutamate dehydrogenase is highly conserved among Clostridium difficile ribotypes.

gluD was highly conserved and glutamate dehydrogenase (GDH) was readily expressed in vitro by all 77 Clostridium difficile ribotypes assayed. All ribotypes, including ARL 002, ARL 027, and ARL 106, were reactive in assays that detect C. difficile GDH.

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Clostridium difficile – From Colonization to Infection

Clostridium difficile is the most frequent cause of nosocomial antibiotic-associated diarrhea. The incidence of C. difficile infection (CDI) has been rising worldwide with subsequent increases in morbidity, mortality, and health care costs. Asymptomatic colonization with C. difficile is common and a high prevalence has been found in specific cohorts, e.g., hospitalized patients, adults in nursi...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2016

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0165579