Copper(II) Complexes as Functional Models for Lytic Polysaccharide Monooxygenase: A Kinetic Report

Mononuclear copper(II) complexes based on the symmetric tridentate N3-donor ligands bis(pyridin-2-yl)methyl)amine (HBPA, L1) and its methylated counterpart, di(2-pyridylmethyl)amine (MeDPA, L2) have been prepared, characterized, demonstrated as functional models for lytic polysaccharide monooxygenase. These disrupt synthetic substrate, such p-nitrophenyl--D-glucopyranoside (-PNPG) into p-nitrophenol (PNP) D-allose via oxidative cleavage LPMOs do in nature. The observed spectroscopic kinetic analysis revealed that reaction proceeds hydroperoxide intermediate, whose electronic spectral signature has appeared at 350 nm absorption spectra with formation rate of 1.61 9.06 × 10-3 s1 respectively 1 2. Especially obtained product was newly 400 nm, indicates 7.52 5.45 s1.for 2 respectively. results affirm ability copper LPMOs.