Cooling storage of ram sperm in presence of antioxidant glutathione

In the process of cryopreservation, semen farm animals can be threatened by many factors, such as oxidative damage, which affect motility and plasma membrane function sperm. As an endogenous antioxidant in animals, glutathione (GSH) significantly improve quality thawed sperm when added to diluent semen. This study was aimed at examining effect GSH (given 0.5 mM, 1.5 mM 5 mM) on viability ram following storage under cooling conditions for several days. reduced negative total progressive (CASA), stability membranes (annexin V/PI) incidence apoptosis/necrosis (Yo-Pro-1/PI) maintained °C 24 h 48 hours. However, this neither reflected penetrating nor fertilising ability (zygotic cleavage rate) examined a heterologous (bovine oocytes sperm) vitro fertilisation test with applied storage. These results indicate that addition extender maintain stored least two To make definite conclusion about functionality (fertilising ability), other concentrations should tested.