Conversion of C14-and cis-or trans-4-H3-L-proline to 4-hydroxy-L-proline and 4-oxo-L-proline by Streptomyces antibioticus.

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Conversion of C14-and cis-or trans-4-H3-L-proline to 4-hydroxy-L-proline and 4-oxo-L-proline by Streptomyces antibioticus.

26 Gerhart, J. C., and A. B. Pardee, in Synthesis and Structure of Macromolecules, Cold Spring Harbor Symposia on Quantitative Biology, vol. 28 (1963), p. 491. 27 Changeux, J. P., in Subunit Structure of Proteins, Brookhaven Symposia in Biology, no. 17 (1964), p. 232. 28 Antonini, E., J. Wyman, M. Brunori, J. F. Taylor, A. Rossi-Fanelli, and A. Caputo, J. Biol. Chem., 239, 907 (1964). 29 Gibson...

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Epimerization of trans-4-hydroxy-L-proline to cis-4-hydroxy-D-proline during acid hydrolysis of collagen.

1. trans-4-Hydroxy-r,-proline is epimerized to &+hydroxy-n-proline under the conditions generally used for the hydrolysis of proteins for analyses of amino acids. 2. The cis epimer elutes from a column of UR-30 resin on a Beckman amino acid analyzer at the same time as threonine and hence would be undetected. 3. About 8% of the trans-4-hydroxy-L-proline in collagen can be epimerized to c&4-hydr...

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The biosynthesis of cis-4-hydroxy-L-proline in sandal (Santalum album L.).

Two isomers of hydroxyproline are present in sandal leaves (Santalum album L.), cis-4-hydroxy-Lproline in the free state (Radhakrishnan & Giri, 1954) and bound trans-4-hydroxy-L-proline, which forms a minor proportion (4%) of total hydroxyproline (Kuttan & Radhakrishnan, 1969). Bound trans-4-hydroxy-L-proline in plants (Lamport, 1965) is presumably synthesized by a mechanism similar to that in ...

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Studies on bound trans-4-hydroxy-L-proline in sandal (Santalum album L.).

1. trans-4-Hydroxy-l-proline was found to occur in the bound state in the leaves of sandal (Santalum album L.), in which large amounts of free cis-4-hydroxy-l-proline are also present. 2. Bound trans-4-hydroxy-l-proline was present, associated mainly with a ;wall' fraction and a ;soluble' fraction roughly in equal proportions. 3. Bound proline is present only in small amounts in the ;soluble' f...

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Process optimization for enhancing production of cis-4-hydroxy-l-proline by engineered Escherichia coli

BACKGROUND Understanding the bioprocess limitations is critical for the efficient design of biocatalysts to facilitate process feasibility and improve process economics. In this study, a proline hydroxylation process with recombinant Escherichia coli expressing L-proline cis-4-hydroxylase (SmP4H) was investigated. The factors that influencing the metabolism of microbial hosts and process econom...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1965

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.54.2.542