منابع مشابه
Statistical evaluation of colocalization patterns in immunogold labeling experiments.
The ultrastructural localization of various antigens in a cell using antibodies conjugated to gold particles is a powerful instrument in biological research. However, statistical or stereological tools for testing the observed patterns for significant clustering or colocalization are missing. The paper presents a method for the quantitative analysis of single or multiple immunogold labeling pat...
متن کاملTheoretical considerations of immunogold labeling of cellulose synthesizing terminal complexes
The sodium dodecyl sulfate (SDS)-solubilized freeze fracture replica labeling (SDS-FRL) technique has been successfully applied to a vascular plant, Vigna angularis [Kimura et a!. 1999, Plant Cell II: 2075 2085] and a bacterium, Acetobaeter xylinum [Kimura et al. 2001, J. Bacteriol. 183: 5668 -5674] for understanding cellulose biosynthesis. However, we do not know yet how many gold particles ca...
متن کاملImmunogold Labeling of Cultured Cells and Virus Particles for Electron Microscopy and Cryo-Electron Microscopy Applications
Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) were first realized in the early 1980s [1] and mid-1990s [2, 3]. Since then both methods have quickly become a powerful tools in structural biology. The major advantage is that they allow for the acquisition of images of biological specimens persevered in their native state after they are plunge-frozen onto carbon-coated ...
متن کاملImmunogold labeling-induced synergy effect for amplified photoelectrochemical immunoassay of prostate-specific antigen.
A new photoelectrochemical immunoassay for prostate-specific antigen (PSA) was successfully developed with high sensitivity via immunogold labeling.
متن کاملA Serial Multiplex Immunogold Labeling Method for Identifying Peptidergic Neurons 1 in Connectomes 2 3
13 14 Electron microscopy-based connectomics aims to comprehensively map synaptic connections in 15 neural tissue. However, current approaches are limited in their capacity to directly assign molecular 16 identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-17 section transmission electron microscopy (ssTEM) to identify multiple peptidergic neurons in a 1...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1993
ISSN: 0022-1554,1551-5044
DOI: 10.1177/41.12.8245435