Controlled Protein Hydrolysis with Immobilized Alkaline Endo-Protease
نویسندگان
چکیده
منابع مشابه
Study on Preparation of Immobilized Alkaline Protease and Its Performance
The nano-Fe3O4 particles were prepared by hydrothermal method. Cellulose-based magnetic Fe3O4 was prepared by adding cellulose solution dissolved in sodium hydroxide / thiourea / urea system. The basic protease was used as the catalyst and glutaraldehyde was used as the crosslinking agent, The production of magnetic recyclable immobilized enzyme can greatly reduce the cost of hydrolysis of slud...
متن کاملProtein hydrolysis by immobilized and stabilized trypsin.
The preparation of novel immobilized and stabilized derivatives of trypsin is reported here. The new derivatives preserved 80% of the initial catalytic activity toward synthetic substrates [benzoyl-arginine p-nitroanilide (BAPNA)] and were 50,000-fold more thermally stable than the diluted soluble enzyme in the absence of autolysis. Trypsin was immobilized on highly activated glyoxyl-Sepharose ...
متن کاملOptimization of Alkaline Protease Production by Streptomyces Ambofaciens in Free and Immobilized Form
Optimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L as carbon source and yeast extract 5 g L as nitrogen source. The ...
متن کاملEndo-exo synergism in cellulose hydrolysis revisited.
Synergistic cooperation of different enzymes is a prerequisite for efficient degradation of cellulose. The conventional mechanistic interpretation of the synergism between randomly acting endoglucanases (EGs) and chain end-specific processive cellobiohydrolases (CBHs) is that EG-generated new chain ends on cellulose surface serve as starting points for CBHs. Here we studied the hydrolysis of ba...
متن کاملAlkaline Hydrolysis of N-acetylolycine
A faster reaction of the cation is expected for the compounds with a free amino group in the acyl moiety. Such an increase was observed for Ala-Ala-pnitroan~lide b.ut not with Ala-p-nitroanilide. Fischer · et al. concluded that a larger inc-rease in k1 with the latter compound was compensated by a reductionin k2/ki. so that the observed k1k2/(k~1 + k2) term remains the same. This assumption of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Applied Biotechnology & Bioengineering
سال: 2017
ISSN: 2572-8466
DOI: 10.15406/jabb.2017.02.00028