Control of Lipid Metabolism in Human Leukocytes

Lipid metabolism in human platelets.

Arachidonate metabolism by human polymorphonuclear leukocytes

Release of arachidonic acid by the membrane phospholipase and metabolism by the 5-lipoxygenase pathway was examined in human polymorphonuclear leukocytes (PMNs). The 5-lipoxygenase pathway is activated when PMNs are given arachidonic acid in ethanol and there is extensive metabolism to 5-hydroxyicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). This activation event was shown to be altere...

Formation of lipid inclusions in normal human leukocytes.

Normal peripheral blood leukocytes left standing at room temperature develop large inclusions which increase in number and size with time of incubation. The formation and nature of these inclusions were investigated. At 0 hour the structures were present in only 1% of the cells, whereas at 48 hr, they were in virtually all neutrophils and monocytes. Ultrastructurally, the globules measured 0.5-...

Lipid patterns in human leukocytes maintained in long-term culture.

The lipid composition of leukocytes maintained in long-term culture was examined in order to clarify the role of immaturity in previously observed differences between normal mature leukocytes and leukemic cells. Cell cultures derived from three types of leukocytes were examined: normal lymphocytes, Burkitt lymphoma, and chronic myelocytic leukemia. Lipid extracts were analyzed for total lipid w...

Carbohydrate Metabolism in Leukocytes

In normal polymorphonuclear leukocytes the occurrence of a high rate of aerobic glycolysis with a concomitant large formation of lactic acid has been demonstrated (1, 2) and the enzymes and intermediates of the Embden-Meyerhof pathway have been isolated and identified (3-5). There are indications for the presence and activity of the Krebs cycle, but the contribution of this cycle to metabolism ...