Continuous Production of Clostridium tetani Toxin

Continuous production of Clostridium tetani toxin.

The continuous production of Clostridium tetani toxin has been carried out in a 1-liter stirred culture vessel for as long as 65 days. Toxin production of approximately 120 flocculating units per ml was maintained with a dilution rate of 0.125 hr(-1), a temperature of 34 C, a pH of 7.4, and the addition to the medium of 0.1 g of potassium chloride per liter. The average minimal lethal intraperi...

Clostridium tetani growth and toxin production in the intestines of germfree rats.

Germfree rats were challenged orally and intrarectally with spores of Clostridium tetani. Although C. tetani spores remained viable in the intestinal tract, they were unable to germinate. Germfree rats were then challenged orally with vegetative cells of C. tetani. Vegetative cells were able to colonize the intestinal tract, replicate, and produce toxin. Tetanus antitoxin, but no tetanus toxin,...

Transferability of toxin production in Clostridium haemolyticum IRP-135

Medium for toxin production by Clostridium perfringens in continuous culture.

A tryptone-salts medium for continuous growth and alpha toxin production of Clostridium perfringens type A in an adapted chemostat is described. In such steady-state cultures, fermentative and biochemical activity of C. perfringens remained unchanged. Toxigenic ability to produce alpha, theta, and nu toxins was preserved.

Comparative pathogenomics of Clostridium tetani

Clostridium tetani and Clostridium botulinum produce two of the most potent neurotoxins known, tetanus neurotoxin and botulinum neurotoxin, respectively. Extensive biochemical and genetic investigation has been devoted to identifying and characterizing various C. botulinum strains. Less effort has been focused on studying C. tetani likely because recently sequenced strains of C. tetani show muc...