Construction of “small-intelligent” focused mutagenesis libraries using well-designed combinatorial degenerate primers
نویسندگان
چکیده
منابع مشابه
Construction of "small-intelligent" focused mutagenesis libraries using well-designed combinatorial degenerate primers.
Site-saturation mutagenesis is a powerful tool for protein optimization due to its efficiency and simplicity. A degenerate codon NNN or NNS (K) is often used to encode the 20 standard amino acids, but this will produce redundant codons and cause uneven distribution of amino acids in the constructed library. Here we present a novel "small-intelligent" strategy to construct mutagenesis libraries ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2012
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113820