Construction of PD-L1-siRNA and IL-2 DNA loading PEI lipid nanoparticles in activating T cells and treating lung cancer

The signal transduction of human programmed death ligand 1 (PD-L1), as an important checkpoint gene in the immune system, plays a key role internal function and survival cancer cells. In this paper, PD-L1-siRNA immunostimulatory IL-2 encoding plasmid DNA loaded by polyethylenimine (PEI) lipid nanoparticles (PEI-LNPs) were constructed. Its therapeutic effect on lung was evaluated, its physical parameters such particle size potential measured. binding capacity, cytotoxicity, apoptosis, cell uptake capacity PD-L1-siRNA/IL-2 DNA-PEI-LNPs tested vitro experiments. down-regulation PD-L1 A549 cells cytokine level co-cultured T also examined. study indicated that PD-L1-siRNA/IL-2-PEI-LNP complexes can provide effective treatment for cells; significant increase IFN-γ TNF-α levels decrease IL-10 confirmed change secreted cytokines. NPs combined with tumor-targeting siRNA/pDNA constructed had ability to target down-regulate expression PD-L1, which realized tumor-specific cytokines, considered be highly safe nanocarriers targeted therapy.