Construction of eukaryotic expression vector of TSLC1 gene

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Construction of eukaryotic expression vector of TSLC1 gene

INTRODUCTION To construct a eukaryotic expression vector of the tumour suppressor in lung cancer 1 (TSLC1) gene, so as to explore the mechanisms of tumour suppression of the gene theoretically. MATERIAL AND METHODS The open reading frame (ORF) of TSLC1 gene was amplified with RT-PCR from normal human foreskin acrobystia, and cloned to pMD19-T simple vector (TA Clone method). The resultant pla...

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ژورنال

عنوان ژورنال: Archives of Medical Science

سال: 2011

ISSN: 1734-1922

DOI: 10.5114/aoms.2011.24124