Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies
نویسندگان
چکیده
منابع مشابه
Base plate mechanics of the barnacle Balanus amphitrite (=Amphibalanus amphitrite).
The mechanical properties of barnacle base plates were measured using a punch test apparatus, with the purpose of examining the effect that the base plate flexural rigidity may have on adhesion mechanics. Base plate compliance was measured for 43 Balanus amphitrite (=Amphibalanus amphitrite) barnacles. Compliance measurements were used to determine flexural rigidity (assuming a fixed-edge circu...
متن کاملSpectral Sensitivity of the Barnacle, Balanus amphitrite
The extracellular ocellar potential was used to evaluate the spectral sensitivity of the ocellus of the barnacle, Balanus amphitrite. Maximum relative sensitivity was at 530-540 nm. Studies with chromatic adapting lights suggest that the receptors contain a single photopigment. The spectra were relatively broader in the dark as compared to the light-adapted state. This effect was shown to be du...
متن کاملSelection of suitable reference genes for real-time PCR studies of early developmental stages of sturgeons
In quantitative real-time PCR, the mRNA level can be quantified in relative terms based on the expression ratio of mRNAs of the target gene and an internal reference gene. Since, an internal standard should be expressed at a constant level among different tissues of an organism at all stages of development, and should be unaffected by the experimental treatment, the stability of different refer...
متن کاملQuantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis
The analysis of differences in gene expression is dependent on normalization using reference genes. However, the expression of many of these reference genes, as evaluated by quantitative RT-PCR, is upregulated in acute pancreatitis, so they cannot be used as the standard for gene expression in this condition. For this reason, we sought to identify a stable reference gene, or a suitable combinat...
متن کاملQuantitative Proteomics Study of Larval Settlement in the Barnacle Balanus amphitrite
Barnacles are major sessile components of the intertidal areas worldwide, and also one of the most dominant fouling organisms in fouling communities. Larval settlement has a crucial ecological effect not only on the distribution of the barnacle population but also intertidal community structures. However, the molecular mechanisms involved in the transition process from the larval to the juvenil...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Molecular Biology
سال: 2009
ISSN: 1471-2199
DOI: 10.1186/1471-2199-10-62