Construction of a thiamin sensor from the periplasmic thiamin binding protein
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چکیده
منابع مشابه
Construction of a thiamin sensor from the periplasmic thiamin binding protein.
This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.
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ژورنال
عنوان ژورنال: Chem. Commun.
سال: 2011
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c0cc04228e