Construction of a Collagen-like Protein Based on Elastin-like Polypeptide Fusion and Evaluation of Its Performance in Promoting Wound Healing

In the healing of wounds, human-like collagen (hCol) is essential. However, collagen-based composite dressings have poor stability in vivo, which severely limits their current therapeutic potential. Based on above, we developed a recombinant fusion protein named hCol-ELP, consists hCol and an elastin-like peptide (ELP). Then, examined physicochemical biological properties hCol-ELP. The results indicated that hCol-ELP exhibited more compact homogeneous lamellar microstructure along with properties, it was found to be significantly superior free hCol. compound demonstrated remarkable capacity induce proliferation migration mouse embryo fibroblast cells (NIH/3T3), as well enhance synthesis human skin fibroblasts (HSF) when tested vitro. significant enhancements rate reduction time required for scab removal, thereby exhibiting scar-free effect. findings provide crucial theoretical foundation implementation dressing fields associated traumatic injuries.