Construction and Evaluation of the Brucella Double Gene Knock-out Vaccine Strain MB6 ?bp26?wboA (RM6)

Objective: Brucellosis is a serious zoonotic infection worldwide. To date, vaccination the most effective measure against brucellosis. This study was aimed at obtaining vaccine strain that has high protective efficacy and low toxicity, allows to be differentiated from infection. Methods: Using homologous recombination, we constructed double gene-deletion Brucella MB6 ?bp26?wboA (RM6) evaluated its characteristics, safety efficacy. Results: The RM6 had good proliferative ability stable biological characteristics in vivo vitro. Moreover, it favorable profile elicited specific immune responses mice sheep. Conclusion: may have substantial practical application value.