Construction and diversity analysis of a murine IgE phage surface display library
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چکیده
منابع مشابه
Puzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملConstruction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary
Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies,...
متن کاملDiversity of the Antibody Response to Tetanus Toxoid: Comparison of Hybridoma Library to Phage Display Library
Monoclonal antibodies are important tools in research and since the 1990s have been an important therapeutic class targeting a wide variety of diseases. Earlier methods of mAb production relied exclusively on the lengthy process of making hybridomas. The advent of phage display technology introduced an alternative approach for mAb production. A potential concern with this approach is its comple...
متن کاملConstruction of a human Fab phage display library from antibody repertoires of osteosarcoma patients.
Osteosarcoma is the commonest type of primary malignant bone tumor, frequently found in adolescents at sites of rapid bone growth. Despite current management protocols, up to half of the patients succumb to this disease. Moreover, there is no well-characterized molecular marker for diagnosis and prognosis. Since phage display methodology allows the selection of human antibody fragments with pot...
متن کاملSchistosoma japonicum: construction of phage display antibody library and its application in the immunodiagnosis of infection.
BACKGROUND A monoclonal antibody would be an effective tool for the detection of circulating antigens in the serum of patients with schistosomiasis, but the traditional way of producing monoclonal antibodies is not cost-effective. The objective of this study was to find a new method for the large-scale production of monoclonal antibodies against Schistosoma japonicum (Sj). METHODS A phage dis...
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ژورنال
عنوان ژورنال: Cell Research
سال: 1997
ISSN: 1001-0602,1748-7838
DOI: 10.1038/cr.1997.17