Construction and Characterization of a Fluorescent Sendai Virus Carrying the Gene for Envelope Fusion Protein Fused with Enhanced Green Fluorescent Protein
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چکیده
منابع مشابه
A highly sensitive assay for proteases using staphylococcal protein A fused with enhanced green fluorescent protein.
Enhanced green fluorescent protein (EGFP) was fused with staphylococcal protein A (SpA) and used as a substrate for proteases. An SpA-EGFP assay was done in three steps: (i) digestion of SpA-EGFP by proteases, (ii) addition of rabbit IgG immobilized on Sepharose beads, and (iii) measurement of the fluorescence intensity of supernatant. The assay was sensitive enough to measure picogram levels o...
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Streptococcus suis serotype 2 (S. suis 2) is an important pathogen, responsible for diverse diseases in swine and humans. To obtain a S. suis 2 strain that can be tracked in vitro and in vivo, we constructed the Egfp-HA9801 recombinant S. suis 2 strain with egfp and spc(r) genes inserted via homologous recombination. To assess the effects of the egfp and spc(r) genes in HA9801, the biochemical ...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2010
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.100511