Constructing ORFeome resources with removable termination codons
نویسندگان
چکیده
منابع مشابه
C. elegans ORFeome version 3.1: increasing the coverage of ORFeome resources with improved gene predictions.
The first version of the Caenorhabditis elegans ORFeome cloning project, based on release WS9 of Wormbase (August 1999), provided experimental verifications for approximately 55% of predicted protein-encoding open reading frames (ORFs). The remaining 45% of predicted ORFs could not be cloned, possibly as a result of mispredicted gene boundaries. Since the release of WS9, gene predictions have i...
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A limited number of non-canonical genetic codes have been described in eukaryotic nuclear genomes. Most involve reassignment of one or two termination codons as sense ones [1-4], but no code variant is known that would have reassigned all three termination codons. Here, we describe such a variant that we discovered in a clade of trypanosomatids comprising nominal Blastocrithidia species. In the...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2006
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112209