Considerations for future environmental DNA accreditation and proficiency testing schemes
نویسندگان
چکیده
Environmental DNA (eDNA) monitoring has revolutionized the way biodiversity is surveyed and been proposed as a reliable method to inform management decisions. Recognition of eDNA techniques tools biosecurity require stringent standards assess sample quality reliability results. Laboratories can have their workflows assessed certified through accreditation be involved in proficiency testing schemes provided test accuracy precision molecular methods. Currently, there only one eDNA-based scheme designed competency laboratories amplifying from Great Crested Newt, Triturus cristatus (Laurenti, 1768) water samples. This test, however, closed currently run by invitation United Kingdom. Given paucity ensure high-quality services, this commentary discusses how future could on detection results for environmental applications. We discuss use tissue-derived synthetic oligonucleotides reference materials, need capacity analytical facilities determine well accurately detecting trace blind samples context which fit purpose designed. To complement development schemes, we provide firsthand accounts lessons learned while developing current Newt scheme. Lastly, highlight limitations standardizing rapidly improving disadvantages standardization an Australian-centered perspective means promote active debate topic testing.
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ژورنال
عنوان ژورنال: Environmental DNA
سال: 2021
ISSN: ['2637-4943']
DOI: https://doi.org/10.1002/edn3.243