منابع مشابه
Mutation Detection by Single Strand Conformation Polymorphism and Heteroduplex Analysis
Single strand conformation polymorphism (SSCP) and heteroduplex analysis (HDA) are two of the most popular electrophoresis-based mutation detection methods. Coupled to DNA amplification of the sequence to be analyzed, these techniques have become the methods of choice for a number of molecular diagnostic laboratories. This can be explained mainly by the numerous advantages, namely their technic...
متن کاملMutation Detection
Since the original description of Helicobacter plvori by Warren and Marshall in 1983 there has been a virtual explosion in interest in this organism. It is now causally linked to chronic gastritis, peptic ulcer disease, gastric mucosa-associated lymphoid tissue lymphoma and possibly gastric cancer. It has proved to be the single most important advance in modern gastroenterology. This book is a ...
متن کاملFluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the P53 gene.
Fluorescent-based single-strand conformation polymorphism (F-SSCP) analysis with capillary electrophoresis (CE) is the most common method for the detection of mutation because of its high sensitivity and resolution. In this study, we prepared an inexpensive linear polyacrylamide (LPA), and successfully applied it to CE-SSCP analysis and tandem CE-SSCP/heteroduplex analysis (HA) of the P53 gene ...
متن کاملHigh sensitive approach for point mutation detection based on electrochemiluminescence.
An electrochemiluminescence-polymerase chain reaction (ECL-PCR) method for point mutation detection has been developed. The target is amplified using a tris (bipyridine) ruthenium (TBR)-labeled forward and a biotinylated reverse primer. The amplification products are digested with specific restriction enzyme, then captured onto streptavidin-coated paramagnetic beads, and detected by measuring t...
متن کاملMicroarray-based mutation detection in the dystrophin gene.
Duchenne and Becker muscular dystrophies (DMD and BMD) are X-linked recessive neuromuscular disorders caused by mutations in the dystrophin gene affecting approximately 1 in 3,500 males. The human dystrophin gene spans>2,200 kb, or roughly 0.1% of the genome, and is composed of 79 exons. The mutational spectrum of disease-causing alleles, including exonic copy number variations (CNVs), is compl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Technical Tips Online
سال: 1998
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70099-0