منابع مشابه
Growth of vaccinia and variola viruses in the one-day-old fertile hen's egg.
The cultivation of viruses in fertile hen's eggs has been widely utilized, providing many advantages for virological studies, since the pioneering work of Woodruff and Goodpasture (1931). In most of such works, however, eggs more than 5 days old have been used. Recently, Yoshino (1956) described a technique for experimental infection of the 1-day-old egg, revealing that such an early embryo sup...
متن کاملMajor neutralizing sites on vaccinia virus glycoprotein B5 are exposed differently on variola virus ortholog B6.
Immunization against smallpox (variola virus) with Dryvax, a live vaccinia virus (VV), was effective, but now safety is a major concern. To overcome this issue, subunit vaccines composed of VV envelope proteins from both forms of infectious virions, including the extracellular enveloped virion (EV) protein B5, are being developed. However, since B5 has 23 amino acid differences compared with it...
متن کاملCharacterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and its variola virus homolog in vitro and in a vaccinia virus mouse protection model.
Three distinct chimpanzee Fabs against the A33 envelope glycoprotein of vaccinia virus were isolated and converted into complete monoclonal antibodies (MAbs) with human gamma 1 heavy-chain constant regions. The three MAbs (6C, 12C, and 12F) displayed high binding affinities to A33 (K(d) of 0.14 nM to 20 nM) and may recognize the same epitope, which was determined to be conformational and locate...
متن کاملJoint Diseases Following Variola
One day in last October I was hurriedly called to see a European child, who was suffering from fever and had a convulsion an hour previously. Three days after characteristic variolar eruptions appeared on the wrist and forehead. The small-pox gradually extended and became confluent. Two days after a younger brother of the child got fever and afterwards small-pox. On the 9th day of his illness t...
متن کاملProtection against lethal vaccinia virus challenge in HLA-A2 transgenic mice by immunization with a single CD8+ T-cell peptide epitope of vaccinia and variola viruses.
CD8(+) T lymphocytes have been shown to be involved in controlling poxvirus infection, but no protective cytotoxic T-lymphocyte (CTL) epitopes are defined for variola virus, the causative agent of smallpox, or for vaccinia virus. Of several peptides in vaccinia virus predicted to bind HLA-A2.1, three, VETFsm(498-506), A26L(6-14), and HRP2(74-82), were found to bind HLA-A2.1. Splenocytes from HL...
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ژورنال
عنوان ژورنال: The Lancet
سال: 1852
ISSN: 0140-6736
DOI: 10.1016/s0140-6736(02)41347-5