Concept note: in vitro multi-enzyme approach to estimate crude protein and amino acid digestibility in grains for broilers

Abstract The knowledge of the precaecal digestibility crude protein (CP) and amino acids (AA) has gained importance. However, since animal welfare is given high priority, experiments are recommended to be reduced or replaced. Thus, in vitro approaches interest for feed evaluation. present study aimed an adaption a multi-enzyme assay. An assay predict CP AA feedstuffs swine was adapted standardised quickly broilers. In assay, conditions gastro-intestinal tract chicken were simulated three steps, representing crop, stomach, small intestine. Samples incubated at 41 °C 30 min buffer solution pH 6.0, 135 with pepsin 2.6 120 pancreatin 6.4. rate disappearance nine different cereal legume grains related their previously determined broiler chicken. Regressions between 42-day old had coefficients determination (R 2 = 0.89). method potential extension calibration using further samples single mixed known recommended.