Complex chimeras to map ligand binding sites of GPCRs
نویسندگان
چکیده
منابع مشابه
Molecular dissection of G protein preference using Gsalpha chimeras reveals novel ligand signaling of GPCRs.
Although only 16 genes have been identified in mammals, several Galpha subunits can be simultaneously activated by G protein-coupled receptors (GPCRs) to modulate their complicated functions. Current GPCR assays are limited in the evaluation of selective Galpha activation, thus not allowing a comprehensive pathway screening. Because adenylyl cyclases are directly activated by G(s)alpha and the ...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2003
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzg045