Completed Chromosomes in Thymine-Requiring Bacillus subtilis Spores
نویسندگان
چکیده
منابع مشابه
Chromosomes in Bacillus subtilis spores and their segregation during germination.
Spores of a thymine-requiring mutant of Bacillus subtilis 168 leucine(-), indole(-), thymine(-)) were uniformly labeled with (3)H-thymidine. These were seeded on thinlayer agar plates where they germinated into long-chained microcolonies. Autoradiograms were used to measure the distribution of labeled deoxyribonucleic acid in the chains of cells, which ranged in length from 2 to 32 cells. Four ...
متن کاملDevelopment of competence in thymine-starved Bacillus subtilis with chromosomes arrested at the terminus.
Tryptophan- and thymine-requiring cells of Bacillus subtilis, emerging from an amino acid starvation treatment which causes arrest of the chromosomes at the terminus, were not transformable. During subsequent incubation in a thymineless medium supplemented with amino acids, the cultures developed competence while retaining chromosome arrest. The competent subpopulation apparently shares the syn...
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A thymine-requiring mutant of Bacillus subtilis strain 168 accumulates short DNA chains after brief pulses with [(3)H]thymidine. Reversion of the thy mutation to thy(+) abolishes the accumulation of short DNA chains, suggesting that the accumulation is related to the thy mutation. The reason for this accumulation has been further investigated by analysis of a mutant with a defective uracil-DNA ...
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A statistical evaluation of viable count procedures utilized for obtaining treatment survival curve data for Bacillus subtilis NCTC 8236 spores is described. Within the various recovery conditions tested, incubation on nutrient agar containing 1% dextrose for 48 hr at 37 C was found to promote the highest count of viable spores surviving a variety of bactericidal treatments involving gamma irra...
متن کاملResporulation of outgrowing Bacillus subtilis spores.
Germinated spores of Bacillus subtilis were incubated in outgrowth medium and tested periodically for capacity to sporulate when suspended in sporulation medium. Concurrent measurements were made of deoxyribonucleic acid (DNA) content and numbers of cell division septa and nucleoids. Sporulation potential is shown to reach a peak at about 110 min at which time the chromosomes are probably well ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.120.2.579-582.1974