Competitive repair pathways in immunoglobulin gene hypermutation
نویسندگان
چکیده
منابع مشابه
Competitive repair pathways in immunoglobulin gene hypermutation.
This review focuses on the contribution of translesion DNA polymerases to immunoglobulin gene hypermutation, in particular on the roles of DNA polymerase eta (Poleta) in the generation of mutations at A/T bases from the initial cytosine-targeted activation-induced cytidine deaminase (AID)-mediated deamination event, and of Polkappa, an enzyme of the same polymerase family, used as a substitute ...
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The immunoglobulin (Ig) repertoire achieves functional diversification through several somatic alterations of the Ig locus. One of these processes, somatic hypermutation (SHM), deposits point mutations into the variable region of the Ig gene to generate higher-affinity variants. Activation-induced cytidine deaminase (AID) converts cytidine to uridine to initiate the hypermutation process. Error...
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I mmunoglobulin (Ig) genes are first modified through the V(D)J (V, variable; D, diversity; J, joining) recombina-tion process in pre–B cells. The Rag1/Rag2-dependent V(D)J recombination generates a large repertoire of B lym-phocytes, each expressing a unique antibody molecule. Rearranged Ig genes are further modified by the somatic hypermu-tation process in activated germinal center B lymphocy...
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To investigate the possible involvement of DNA repair in the process of somatic hypermutation of rearranged immunoglobulin variable (V) region genes, we have analyzed the occurrence, frequency, distribution, and pattern of mutations in rearranged Vlambda1 light chain genes from naive and memory B cells in DNA repair-deficient mutant mouse strains. Hypermutation was found unaffected in mice carr...
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To test the hypothesis that immunoglobulin gene hypermutation in vivo employs a pathway in which DNA breaks are introduced and subsequently repaired to produce mutations, we have used a PCR-based assay to detect and identify single-strand DNA breaks in lambda1 genes of actively hypermutating primary murine germinal center B cells. We find that there is a two- to threefold excess of breaks in la...
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ژورنال
عنوان ژورنال: Philosophical Transactions of the Royal Society B: Biological Sciences
سال: 2008
ISSN: 0962-8436,1471-2970
DOI: 10.1098/rstb.2008.0206