Comparison of Various Methods for Preparation of Viral Serological Antigens from Infected Cell Cultures

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Comparison of various methods for preparation of viral serological antigens from infected cell cultures.

In efforts to prepare more potent and sensitive viral serological antigens, several aspects of the production of antigens from infected cell cultures were studied. Antigens derived from whole, infected culture material and from the cellular and fluid phases were compared. Freezing and thawing, sonication, and alkaline buffer extraction were compared for effectiveness in releasing antigen from h...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1971

ISSN: 0003-6919

DOI: 10.1128/am.21.2.217-226.1971