Comparison of Transforming Growth Factor 1 (TGF-β1) Expression in Various Lysate Platelet-Rich Fibrin (L-PRF) Concentrations on Human Dental Pulp Stem Cell Differentiation

Objective: To compare Transforming growth factor-β1 (TGF-β1) expression in various L-PRF concentrations on the hDPSC differentiation process. Material and Methods: cell cultures were subjected to serum starvation by reducing FBS levels culture media. Lysate PRF was obtained from gel, which then incubated at 4°C for 24 h. The supernatant dried, transferred a 2-ml Eppendorf tube, stored −20°C. evaluation of TGF-β1 1%, 5%, 10%, 25% samples 10% (control) during process quantified using an ELISA reader day 7. one-way ANOVA test, followed Bonferroni’s post hoc test with significant values (p<0.05). Results: Significant differences noted between control group (10% FBS). highest occurred (0.645 ± 0.048), (0.461 0.035), (0.374 0.013), 5% (0.275 0.045), lowest 1% (0.160 0.045). Conclusion: best result TGF-B1 group.