Comparison of Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Assay and Enzyme Assay for Diagnosis of GM1-Gangliosidosis in Shiba Dogs
نویسندگان
چکیده
منابع مشابه
Polymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs
Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained ...
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GM1 gangliosidosis is characterized by a deficiency in the lysosomal hydrolase beta-galactosidase, progressive nervous system disease and ocular lesions. Diagnosis of GM1 gangliosidosis in humans and cats with the analogous disease has been made by measurement of the enzyme activity in various tissues including brain, liver and cultured skin fibroblasts. The authors report the use of cultured c...
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MicroRNA (miRNA) studies are experiencing a transition from basic research applications to clinical applications. However, the lack of reliable and sensitive miRNA detection methods has become a bottleneck in the process. Here, we report an absolute quantification method based on the competitive PCR amplification of specific miRNAs and synthetic RNA spike-ins in a single reaction. RNA spike-ins...
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BACKGROUND:Avian reoviruses (ARVs) are members of theOrthoreovirus genus; one of the 12 genera of the Reoviridaefamily. The ARVs are the cause of some important diseases inpoultry such as reovirus-induced arthritis, tenosynovitis,chronic respiratory disease, and mal-absorption syndrome.OBJECTIVES: In this study, the presence of ARVs in the Iranianbreeder flocks was investigated through reverse ...
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Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for epidemiologic studies. The present study was performed with a commercial human en...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2004
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063870401600407