Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat
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چکیده
منابع مشابه
Identification of Low Molecular Weight Glutenin Alleles by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) in Common Wheat (Triticum aestivum L.)
Low molecular weight glutenin subunits (LMW-GS) play an important role in determining dough properties and breadmaking quality. However, resolution of the currently used methodologies for analyzing LMW-GS is rather low which prevents an efficient use of genetic variations associated with these alleles in wheat breeding. The aim of the current study is to evaluate and develop a rapid, simple, an...
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Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the Glu-A3 locus, thirteen c, twelve d, three e (null), two g and two new alleles were identified. At the G...
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in this study, 110 samples of bread wheat, aegilops crassa, ae. cylindrica and ae. tauschii were investigated to identify low-molecular-weight glutenin subunits. overall, the four alleles in term of the analysis of sds-page gels were detected. the ʻaʼ allele with 45% has the greatest frequency, and after that, ʻbʼ, ʻcʼ and ʻdʼ alleles were with 38.1%, 11.8% and 9.1% frequencies, respectively; h...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2010
ISSN: 1471-2229
DOI: 10.1186/1471-2229-10-124