Comparison of large-insert, small-insert and pyrosequencing libraries for metagenomic analysis
نویسندگان
چکیده
منابع مشابه
Assembling short reads from jumping libraries with large insert sizes
MOTIVATION Advances in Next-Generation Sequencing technologies and sample preparation recently enabled generation of high-quality jumping libraries that have a potential to significantly improve short read assemblies. However, assembly algorithms have to catch up with experimental innovations to benefit from them and to produce high-quality assemblies. RESULTS We present a new algorithm that ...
متن کاملStrong spurious transcription likely a cause of DNA insert bias in typical metagenomic clone libraries
Background: Clone libraries provide researchers with a powerful resource with which to study nucleic acid from diverse sources. Metagenomic clone libraries in particular have aided in studies of microbial biodiversity and function, as well as allowed the mining of novel enzymes for specific functions of interest. These libraries are often constructed by cloning large-inserts (~30 kb) into a cos...
متن کاملStrong spurious transcription likely contributes to DNA insert bias in typical metagenomic clone libraries
BACKGROUND Clone libraries provide researchers with a powerful resource to study nucleic acid from diverse sources. Metagenomic clone libraries in particular have aided in studies of microbial biodiversity and function, and allowed the mining of novel enzymes. Libraries are often constructed by cloning large inserts into cosmid or fosmid vectors. Recently, there have been reports of GC bias in ...
متن کاملSequence analysis Assembling short reads from jumping libraries with large insert sizes
Motivation: Advances in Next-Generation Sequencing technologies and sample preparation recently enabled generation of high-quality jumping libraries that have a potential to significantly improve short read assemblies. However, assembly algorithms have to catch up with experimental innovations to benefit from them and to produce high-quality assemblies. Results: We present a new algorithm that ...
متن کاملConstruction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences.
We describe an efficient method for the construction of small-insert genomic libraries enriched for highly polymorphic, simple sequence repeats. With this approach, libraries in which 40-50% of the members contain (CA)n repeats are produced, representing an approximately 50-fold enrichment over conventional small-insert genomic DNA libraries. Briefly, a genomic library with an average insert si...
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ژورنال
عنوان ژورنال: The ISME Journal
سال: 2012
ISSN: 1751-7362,1751-7370
DOI: 10.1038/ismej.2012.35