Comparison of immunotyping of Chlamydia trachomatis by indirect fluorescent-antibody staining and radioimmunoassay
نویسندگان
چکیده
منابع مشابه
Comparison of rabies humoral antibody titers in rabbits and humans by indirect radioimmunoassay, rapid-fluorescent-focus-inhibition technique, and indirect fluorescent-antibody assay.
Rabies humoral antibodies were induced in eight New Zealand rabbits by a single intramuscular injection of inactivated suckling mouse brain rabies vaccine. The primary response to immunization was measured in blood samples taken at selected intervals for 6 months. The anamnestic response was measured in blood samples obtained 2 weeks after the rabbits received a booster immunization. The humora...
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SUMMARY The results of direct stool examination in 102 watery and bloody diarrhea patients were compared with the presence of antibodies against Entamoeba histolytica with Indirect Fluorescent Antibody Test. Besides 22 healthy individuals included in this study as control group. It was found that 22 patients (21.5%) with a sera titre higher than 1/320 bad only 9 (8.8%) positive E. Histolytica ...
متن کاملInfluence of endocervical specimen adequacy on PCR and direct fluorescent-antibody staining for detection of Chlamydia trachomatis infections.
The cellular quality of the endocervical swab specimen used for the detection of Chlamydia trachomatis may dramatically impact the sensitivity of the diagnostic assay used. An evaluation of the adequacy of 319 endocervical swab specimens from women attending two inner-city sexually transmitted disease and family planning clinics, as well as five high school-based family planning clinics, was pe...
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Chlamydia trachomatis is an obligate intracellular bacterium which causes a wide variety of human infections such as ocular, urogenital and respiratory infections. Genital infections of women, espe-cially when repeated, give rise to many complications such as ectopic pregnancy, miscarriage and infertility. Since chlamydial infections are usually asymptomatic, they progress unnoticed and produc...
متن کاملIndirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies.
Indirect immunofluorescence (IF) staining, using a monoclonal antibody, detected two- to fourfold more inclusions than did iodine staining. Of 274 clinical specimens, 53 (19.3%) were positive by IF on passage 1 as compared with 33 (12%) by iodine staining (P less than 0.005). IF-stained inclusions in McCoy cells in the bottom of microculture wells were readily viewed with a long-focal-length ob...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1992
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.30.4.1014-1015.1992