Comparison of DNA fingerprinting and serotyping for identification of avian Pasteurella multocida isolates

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA

DNA fingerprinting of Pasteurella multocida recovered from avian sources.

Repetitive sequence-based PCR (rep-PCR) and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field isolates and 4 attenuated vaccine strains of Pasteurella multocida recovered from multiple avian species. Both rep-PCR and AFLP assays were rapid and reproducible, with high indices of discrimination. Concordance analyses of rep-PCR and AFLP with somatic serot...

characterization of avian pasteurella multocida isolates by protein profiles and restriction enzyme analysis of chromosomal dna

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Immune efficacy of OmpH and OmpA DNA vaccines against avian Pasteurella multocida

Avian Pasteurella multocida is an agent of fowl cholera. The protective effect achieved through orthodoxvaccines is not ideal. The research on novel vaccines against avian Pasteurella multocida is imperative. Inthis study, the genes encoding outer membrane protein H and A (OmpH and OmpA) were cloned into theeukaryotic expression vector pcDNA3.1(+) and the recombinant plasmids, namely DNA vaccin...

Isolation, Identification and Antibiogram of Pasteurella Multocida Isolates of Avian Origin

Isolation of Pasteurella multocida was attempted from the heart blood, spleen, liver and lung collected from turkeys (4 No,s) and chicken (4 No,s) suspected to have died of avian pasteurellosis. A total of eight P. multocida isolates were isolated and identified on the basis of biochemical characteristics, pathogenicity studies in mice and PM-PCR. The In vitro antibiotic sensitivity test of the...